---------------------------------------------------------------------------- SUMMARY TABLES These tables 'summarize' the expression data for all of the signatures found in our libraries. To determine the normalized abundance value for each signature in the library, we separately merged 2-step runs and 3-step runs to create a raw abundance count for each stepper. The raw abundance count was calculated as the average from all 2-step or from all 3-step runs for a given signature. The average total number of signatures sequenced in all 2-step or for all 3-step runs was also calculated within the library. The final stage to merge the data within the steppers was to calculate the normalized abundance for each stepper. The normalized abundance is the raw abundance count divided by the average total number of signatures for both of the steppers in the library, multiplied by 250,000 to obtain a "transcripts per quarter million" or TPQ value. The above method was applied separately for signatures matching to tRNAs, rRNAs, snRNAs or snoRNAs. These signatures were removed from the primary set of small RNA signatures and these data are in a different file (smallRNA_17_trsnosn_summary.txt). ---------------------------------------------------------------------------- COLUMN HEADINGS The summary table includes the following columns: Signature - 17nt smallRNA sequence Hits - is the number of occurrences of the signatures in the Arabidopsis genome FLR, etc. - normalized data - see key to libraries on our web page. These values are normalized abundance, in transcripts per quarter (TPQ). The value was derived by a series of steps to merge the sequencing runs, described in above and in more detail in Meyers et al. (2004, Genome Research 14:1641) or Lu et al. (2005). ---------------------------------------------------------------------------- Questions regarding these data should be directed to Blake Meyers (meyers@dbi.udel.edu).